Genetically-engineered whole-cell bioreporters on integrated circuits for very low-level chemical sensing

Author

Islam, S.K. ; Weathers, B. ; Terry, S.C. ; Zhang, M. ; Blalock, B. ; Caylor, S. ; Ripp, S. ; Sayler, G.S.

Author_Institution

Dept. of Electr. & Comput. Eng., Tennessee Univ., Knoxville, TN, USA

fYear

2005

fDate

12-16 Sept. 2005

Firstpage

351

Lastpage

354

Abstract

We report a biosensor using genetically-engineered whole-cell bioreporters on integrated circuit for low-level chemical sensing. The bioluminescent bioreporters are bacteria that can be genetically altered to achieve bioluminescence when in contact with a targeted substance. The bioreporters are placed on a microluminometer. The microluminometer includes integrated photodetector and signal processor and is realized on a standard CMOS process. The bioluminescent bioreporter integrated circuit (BBIC) can detect luminescence from as few as 5000 fully induced pseudomonas fluorescene 5RL bacteria cell.

Keywords

CMOS integrated circuits; bioluminescence; biosensors; chemical sensors; digital signal processing chips; genetic engineering; microorganisms; photodetectors; CMOS; bioluminescence; bioluminescent bioreporter integrated circuit; biosensor; chemical sensor; genetic engineering; integrated photodetector; microluminometer; pseudomonas fluorescene 5RL bacteria cell; signal processor; Bioluminescence; Chemical technology; Fluorescence; Microorganisms; Organisms; Pollution measurement; Proteins; Signal analysis; Signal generators; Soil;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Device Research Conference, 2005. ESSDERC 2005. Proceedings of 35th European

Print_ISBN

0-7803-9203-5

Type

conf

DOI

10.1109/ESSDER.2005.1546657

Filename

1546657