Does Background Intensity Estimation Influence the Iterative Restoration of Microscope Images?

Author

Ponti, Moacir P., Jr. ; Mascarenhas, Nelson D A

Author_Institution

Inst. de Cienc. Mat. e de Comput. (ICMC), Univ. de Sao Paulo (USP), São Carlos, Brazil

fYear

2010

fDate

Aug. 30 2010-Sept. 3 2010

Firstpage

56

Lastpage

62

Abstract

A new background estimation method is proposed to improve iterative restoration of microscope images. The method is based on a sequence of subtractions performed in the beginning of an iterative restoration procedure. A series of experiments using background intensity detection methods were carried out in order to analyse the influence of a correct estimation of the background. The restoration results as a function of the detected background value is discussed. The results indicates a strong influence of the estimate on the restoration of images. When compared to an optimal error-based method, the proposed method was faster and obtained a good approximation to the correct value.

Keywords

image restoration; iterative methods; object detection; background intensity detection methods; background intensity estimation; iterative restoration procedure; microscope images; optimal error-based method; subtraction sequence; Biomedical imaging; Estimation; Histograms; Image restoration; Iterative methods; Microscopy; Noise; background estimation; image restoration; microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Graphics, Patterns and Images (SIBGRAPI), 2010 23rd SIBGRAPI Conference on

Conference_Location

Gramado

Print_ISBN

978-1-4244-8420-1

Type

conf

DOI

10.1109/SIBGRAPI.2010.16

Filename

5720347