FTIR/ATR Spectroscopy Applied to the Rapid Analysis for Hemoglobin in Human Soluble Blood Samples

Author

Tao, Pan ; Aihong, Peng ; Wenjie, Huang ; Hao, Yin ; Yun, Han

Author_Institution

Key Lab. of Optoelectron. Inf. & Sensing Technol., Jinan Univ., Guangzhou, China

Volume

2

fYear

2011

fDate

6-7 Jan. 2011

Firstpage

268

Lastpage

271

Abstract

By directly measuring human soluble blood samples by Fourier transform infrared spectroscopy (FTIR) and attenuated total reflection (ATR) technology, the rapid quantitative analysis method for human hemoglobin (HGB) was established. The optimization of Savitzky-Golay (SG) smoothing modes combined with partial least squares (PLS) factor was applied to optimize the model of FTIR/ATR spectroscopy analysis here. The optimal smoothing parameters were 1st order derivative smoothing, 2nd degree polynomial, 59 smoothing points, the optimal PLS factor, RMSEP, RP and RRMSEP were 8, 4.21g/L, 0.962 and 3.1% respectively, which was obviously superior to the direct PLS model without SG smoothing. This demonstrated that FTIR/ATR spectroscopy can be applied to the rapid determination of human hemoglobin concentration, large-scale simultaneous optimization selection of SG smoothing parameters and PLS factor can be effectively applied to the model optimization of FTIR/ATR analysis.

Keywords

Fourier transform spectra; attenuated total reflection; blood; infrared spectra; least squares approximations; medical signal processing; molecular biophysics; optimisation; proteins; smoothing methods; ATR spectroscopy; FTIR spectroscopy; Fourier transform infrared spectroscopy; Savitzky-Golay smoothing modes; attenuated total reflection; hemoglobin; human hemoglobin; human soluble blood samples; optimization; partial least squares factor; Blood; Humans; Optimization; Polynomials; Predictive models; Smoothing methods; Spectroscopy; FTIR/ATR; Hemoglobin; PLS; SG smoothing; human soluble blood sample;

fLanguage

English

Publisher

ieee

Conference_Titel

Measuring Technology and Mechatronics Automation (ICMTMA), 2011 Third International Conference on

Conference_Location

Shangshai

Print_ISBN

978-1-4244-9010-3

Type

conf

DOI

10.1109/ICMTMA.2011.354

Filename

5721172