A New Algorithm for Computing Infarct Volume in a Rat Stroke Model

There are several methods for computing the infarct volume from brain images of stroke animal models. However, most methods rely on the discretion of the user to detect the infarct region and do not differentiate the levels of infarction within the infarct region when computing the infarct volume. We have developed a new algorithm for detecting the infarct region in rat brain slices stained with the triphenyltetrazolium chloride (TTC) and for computing the infarct volume in proportional to infarction levels. Comparison of the algorithm with other methods shows that it computes the infarct volume more accurately and efficiently than other methods without requiring the user intervention.