Microrobotically Fabricated Biological Scaffolds for Tissue Engineering

Author

Nain, Amrinder S. ; Chung, Franklin ; Rule, Michael ; Jadlowiec, Julie A. ; Campbell, Phil G. ; Amon, Cristina ; Sitti, Metin

Author_Institution

Inst. for Complex Engineered Syst., Carnegie Mellon Univ., Pittsburgh, PA

fYear

2007

fDate

10-14 April 2007

Firstpage

1918

Lastpage

1923

Abstract

A microrobotic method for fabricating multilayered poly(lactic acid) (PLA) biological scaffolds using micropipettes for tissue engineering applications is presented. Biological scaffolds are fabricated over several different substrates by drawing and solidification of a viscous liquid polymer solution pumped continuously through a glass micropipette. The proposed method produces highly aligned, multilayered, crisscrossed fiber scaffolds with user specified pore sizes and diameters in the range from 1 to 10 micrometer. Attachment, proliferation and differentiation of C2C12 mouse pluripotential cells seeded on individual, parallel, and intersecting fibers is successfully demonstrated. The proposed robotic methodology consistently provides parameterized biological scaffolds to aid studies in tissue engineering and to develop novel MEMS, filtration and controlled drug delivery devices

Keywords

biological tissues; drug delivery systems; medical robotics; microrobots; tissue engineering; C2C12 mouse pluripotential cells; MEMS; cell attachment; cell differentiation; cell proliferation; drug delivery devices; glass micropipette; micropipettes; microrobotically fabricated biological scaffolds; multilayered crisscrossed fiber scaffolds; multilayered polylactic acid biological scaffolds; tissue engineering; viscous liquid polymer solution; Engineering drawings; Filtration; Glass; Mice; Micromechanical devices; Optical fiber devices; Polymers; Programmable logic arrays; Robots; Tissue engineering;

fLanguage

English

Publisher

ieee

Conference_Titel

Robotics and Automation, 2007 IEEE International Conference on

Conference_Location

Roma

ISSN

1050-4729

Print_ISBN

1-4244-0601-3

Electronic_ISBN

1050-4729

Type

conf

DOI

10.1109/ROBOT.2007.363602

Filename

4209366