Title :
Super-resolution tracking of weak fluorescent markers in 3D: application in dissecting the mechanics of chromosome segregation using fluorescence imaging and molecular genetics in yeast
Author :
Danuser, Gaudenz
Author_Institution :
Laboratory for Comput. Cell Biol., The Scripps Res. Inst., La Jolla, CA, USA
Abstract :
We present a framework for the tracking of fluorescent tags in 4D microscopy (3D + time). It includes algorithms to separate tag signals under strong interference, allowing us to break the limits of optical resolution in light microscopy by a factor 3. We apply the package to read out chromosome dynamics in a functional, cell-based screen for proteins regulating cell division in yeast.
Keywords :
biological techniques; cellular biophysics; fluorescence; genetics; molecular biophysics; optical microscopy; proteins; 4D microscopy; cell division; chromosome dynamics; chromosome segregation; fluorescence imaging; fluorescent tags; light microscopy; molecular genetics; optical resolution; proteins; super-resolution tracking; weak fluorescent markers; yeast; Biological cells; Fluorescence; Fungi; Genetics; Image resolution; Interference; Optical imaging; Optical microscopy; Packaging; Signal resolution;
Conference_Titel :
Biomedical Imaging: Nano to Macro, 2004. IEEE International Symposium on
Print_ISBN :
0-7803-8388-5
DOI :
10.1109/ISBI.2004.1398474
