Title :
Fluorescence nanoscopy: breaking the diffraction barrier by the RESOLFT concept
Author_Institution :
Dept. of NanoBiophotonics, Max Planck Inst. for Biophys. Chem., Gottingen, Germany
Abstract :
In this lecture, principle of fundamentally breaking the diffraction barrier through reversible saturable optical (fluorescence) transitions (RESOLFT) is discussed. In these concepts, the diffraction barrier is broken by a saturated optical transition (depletion) between two states of a marker, whereby the transition is effected with an intensity distribution featuring one or more intensity minima (zero). The saturation level defines the size of the ultrasharp focal spot and/or the concomitantly enlarged bandwidth of the optical transfer function (OTF).
Keywords :
fluorescence; light diffraction; nanotechnology; optical microscopy; optical saturation; optical transfer function; RESOLFT; diffraction barrier; fluorescence nanoscopy; optical transfer function; reversible saturable optical fluorescence transitions; saturated optical transition; Biomedical optical imaging; Chemistry; Fluorescence; Microscopy; Optical diffraction; Optical saturation; Optimized production technology; Spatial resolution; Stationary state; Stimulated emission;
Conference_Titel :
Lasers and Electro-Optics Society, 2005. LEOS 2005. The 18th Annual Meeting of the IEEE
Print_ISBN :
0-7803-9217-5
DOI :
10.1109/LEOS.2005.1547859
