Synthesis and material properties of magnetic fluid coated with pegylated chondroitin sulfate

We tried to fabricate a magnetic fluid coated with chondroitin sulfate having "stealth" function by PEGylation of chondroitin sulfate (CS). PEGylation of chondroitin sulfate was carried out by polymerizing polyethylene glycol (PEG) and CS. Carboxyl group contained within chondroitin sulfate and hydroxyl group within PEG was esterized by condensation agent. The obtained samples were analyzed by infrared spectroscopic (IR) analysis and nuclear magnetic resonance (NMR) analysis. Those analysis results confirmed that the samples were PEGylated CS. Composite of PEGylated CS and magnetic particles was attempted. The synthesis of magnetic fluid coated with PEGylated chondroitin sulfate (PEG-MFCS) was applied the coprecipitation method. Our previous researches confirmed the properties of Magnetic fluid coated with saccharides were affected by functional group contained within saccharides. So, the properties of PEG-MFCS were investigated for revealing the effects of PEGylating to chondroitin sulfate on them. The measurement of hydrodynamic diameters, magnetic particle diameters and saturation magnetization was carried out. Then, X-ray crystal structural analysis was carried out for investigation of magnetic particle\´s crystal structure. As a result, PEGylated chondroitin sulfate didn\´t have a much greater influence on the characterizations of PEG-MFCS. It was succeeded to synthesize PEG-MFCS without loss of functionality.