Title :
Quantitative analysis of volumetric TPLSM data
Author :
Ifarraguerri, Agustin ; Thompson, Beverly ; Tsai, Philbert S. ; Friedman, Beth ; Schaffer, Chris B. ; Lev-Ram, Varda ; Tsien, Roger Y. ; Squier, Jeff A. ; Kleinfeld, David
Author_Institution :
Science Applications Int. Corp., Arlington, VA, USA
Abstract :
This paper describes initial algorithm development in support of research on a new all-optical histology technique based on two-photon laser scanning microscopy (TPLSM) and laser tissue ablation. This new imaging modality makes it possible to obtain quantitative information that can be used to measure physiological parameters of interest. We present our initial image processing approach and volumetric rendering results. Two examples of useful quantitative parameters are presented: measurement of blood vessel volume fraction and cell counting.
Keywords :
biomedical optical imaging; blood vessels; cellular biophysics; laser applications in medicine; medical image processing; optical microscopy; rendering (computer graphics); two-photon processes; all-optical histology; blood vessel volume fraction; cell counting; image processing; laser tissue ablation; volumetric two-photon laser scanning microscopy data; Biomedical imaging; Blood vessels; Fluorescence; Image reconstruction; Image segmentation; Laser ablation; Laser beam cutting; Laser theory; Microscopy; Rendering (computer graphics);
Conference_Titel :
Biomedical Imaging: Nano to Macro, 2004. IEEE International Symposium on
Print_ISBN :
0-7803-8388-5
DOI :
10.1109/ISBI.2004.1398660
