Penalty/modified barrier function method for diagnostic imaging using area and point illumination geometries in fluorescence-enhanced optical tomography

A novel imaging technique is developed for (i) area illumination and area collection and (ii) point illumination and point collection geometries in 3D fluorescence-enhanced optical tomography. The target reconstruction problem was formulated as a solution to a nonlinear least-squares-type simple bounds constrained optimization problem. The numerical technique for target reconstruction is based on the penalty/modified barrier function method and the parameter estimates were minimized by the gradient based constrained truncated Newton with trust region method. Three dimensional targets were reconstructed from experimental data under two different experimental conditions of (i) perfect uptake (1:0, target to background ratio) and (ii) imperfect uptake (100:1, target to background ratio).