Title :
Detection and tracking of rolling leukocytes from intravital microscopy
Author :
Acton, Scott T. ; Ray, Nilanjan
Author_Institution :
Dept. of Electr. & Comput. Eng. & Biomed. Eng., Virginia Univ., Charlottesville, VA, USA
Abstract :
We present a method for detecting and tracking rolling leukocytes from intravital video microscopy. The paper emphasizes a new method for detecting cells that is based on finding ellipses that have maximal outward normal gradient and minimal gradient variance around the boundary. A fast convolution-based implementation of the detector is discussed. For tracking, we present a radial active model that exploits a gradient vector flow field tailored to tracking objects in the direction of blood flow. Results are presented that compare the detection performance and tracking performance to that of existing methods.
Keywords :
biomedical optical imaging; cellular biophysics; convolution; haemodynamics; optical microscopy; blood flow direction; cell detection; convolution; ellipses finding; gradient vector flow field; intravital video microscopy; maximal outward normal gradient; minimal gradient variance; object tracking; radial active model; rolling leukocytes detection; rolling leukocytes tracking; Biological system modeling; Biomedical computing; Biomedical engineering; Blood flow; Decision support systems; Detectors; In vivo; Microscopy; Statistics; White blood cells;
Conference_Titel :
Biomedical Imaging: Nano to Macro, 2004. IEEE International Symposium on
Print_ISBN :
0-7803-8388-5
DOI :
10.1109/ISBI.2004.1398768
