DocumentCode :
2638799
Title :
Dual capillary electrophoresis devices with electrochemical detection on a single platform
Author :
Dorairaj, Rathissh ; Roussel, Thomas J. ; Crain, Mark M. ; Jackson, Douglas J. ; Franco, Danielle ; Walsh, Kevin M. ; Baldwin, Richard P. ; Naber, John F. ; Keynton, Robert S.
Author_Institution :
Dept. of Electr. & Comput. Eng., Louisville Univ., KY, USA
fYear :
2005
fDate :
12-15 May 2005
Firstpage :
15
Lastpage :
17
Abstract :
The purpose of this paper is to demonstrate the feasibility of developing a single lab-on-a-chip (LOC) platform capable of performing dual, simultaneous separation and detection of multiple analytes. Computational modeling was performed to determine optimum device geometry and performance. The soda-lime glass-based device was fabricated using traditional microtechnology processes, including UV photolithography, buffered oxide etch (BOE), electrode deposition and compression thermal bonding. The device was characterized with a mixture of dopamine (2mM) and catechol (2mM) in a phosphate buffer (20mM, 6.5 pH). Modeling results yielded migration velocities of 0.6 mm/s and 0.42 mm/s for dopamine (electrokinetic (EK) mobility=60,000 μm2/V·s) and catechol (EK mobility=42,000 μm2/V·s), respectively. Experimental results obtained from microchips exhibiting the same EK mobilities demonstrated identical electropherograms in both detection channels with migration velocities of 0.58 mm/s for dopamine and 0.41 mm/s for catechol.
Keywords :
biochemistry; biological techniques; calcium compounds; electrochemical analysis; electrokinetic effects; electrophoresis; etching; micromechanical devices; silicon compounds; sodium compounds; ultraviolet lithography; Na2O-CaO-SiO2; UV photolithography; buffered oxide etch; catechol; compression thermal bonding; computational modeling; dopamine; dual capillary electrophoresis device; electrochemical detection; electrode deposition; electrokinetic mobility; electropherogram; lab-on-a-chip platform; microtechnology process; migration velocity; multiple analyte; optimum device geometry; phosphate buffer; soda-lime glass-based device fabrication; Biochemical analysis; Computational geometry; Computational modeling; Electrodes; Electrokinetics; Glass; Lab-on-a-chip; Plugs; Reservoirs; Voltage;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on
Print_ISBN :
0-7803-8711-2
Type :
conf
DOI :
10.1109/MMB.2005.1548371
Filename :
1548371
Link To Document :
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