Automated multiplexed multidensity microfluidic (M3) cell sensing based on electrical gain measurements

We present the preparation of multi-density cells automated in a microfluidic channel and then sensed with multiplexed surface microelectrode arrays at the bottom of the channel. Decreasing concentrations of cells are prepared by mixing decreasing volumes of cells with increasing volumes of cell culture media. The different cell concentrations are separated by oil blocks. The fluids are driven into the channel by three computer-controlled syringe pumps and Bode plot of the network formed the cells are generated continuously. The monitoring of the cells is carried out as the maxima of the Bode plot are plotted with respect to time showing the kinetics of cell growth. As an initial result, this monitoring is done for three sets of concentration of cells using glass/PDMS wells simultaneously. Preparation of different densities of cells is carried out by adjusting the ration of flow of the cells and media in a microchannel using a computer controlled syringe pumps system. For the conference, we will present the growth patterns of 16 density cells in order to study the effect of initial concentration on the cell growth of three different types of cells.