Title :
Application of a novel widefield surface plasmon microscope in binding assays
Author :
Marlafeka, S. ; Liu, S. ; Jamil, M. Mahadi Abdul ; Twigg, P.C. ; Britland, S.T. ; Denyer, M.C.T. ; See, C.W. ; Somekh, M.G.
Author_Institution :
Pharmacy Dept., Bradford Univ., UK
Abstract :
The present work investigates the capabilities of a novel widefield surface plasmon microscope (WSPR) and its potential applications in biology. Here in particular, we concentrate on imaging interfacial interactions between specific antibodies and the proteins laminin and fibronectin, both patterned onto prefabricated Au/Cr/glass substrates. These experiments are used to demonstrate the high sensitivity, good contrast and high lateral resolution (close to a micron) of the new system. These results indicate that our microscope may form the basis for a new generation of fast assay systems.
Keywords :
biochemistry; biological techniques; cellular biophysics; molecular biophysics; proteins; surface plasmon resonance; WSPR; biology; fabricated Au-Cr-glass substrates; fast assay systems; fibronectin; imaging interfacial interaction; potential application; proteins laminin; surface plasmon microscope; Atomic force microscopy; Chromium; Glass; Gold; High-resolution imaging; Immune system; Optical imaging; Pharmaceuticals; Plasma temperature; Plasmons; cell; immunoassay; surface plasmon; widefield;
Conference_Titel :
Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on
Print_ISBN :
0-7803-8711-2
DOI :
10.1109/MMB.2005.1548406