Co-culture of cells in PDMS microsystem for sensitized artificial skin

Author

Griscom, Laurent ; Chateau, Yannick ; Pennec, Jean-Pierre ; Misery, Laurent ; Le Pioufle, Bruno

Author_Institution

Ecole Normale Superieure de Cachan, Bruz, France

fYear

2005

fDate

12-15 May 2005

Firstpage

184

Lastpage

187

Abstract

In this work a PDMS microsystem was conceived and fabricated for the development and study of an artificial skin-equivalent that includes nerve endings. A cellular co-culture of keratinocytes, dorsal root ganglia neurons and spinal cord cells in micro wells was developed. Microstructures were included in the microsystems, allowing infiltration of neuronal extensions to the skin-equivalent compartment. Electrophysiological measurements were conducted to measure electrical responses of the neurons in response to external stimulation of the skin.

Keywords

bioelectric phenomena; biomedical transducers; cellular biophysics; micromechanical devices; neurophysiology; polymers; skin; tissue engineering; PDMS microsystem; artificial skin-equivalent; biomedical transducers; dorsal root ganglia neurons; electrophysiological measurement; external electric stimulation; keratinocyte cell co-culture; micro wells; microstructures; nerve endings; nervous system; neuronal electrical response measurement; neuronal extension infiltration; sensitized artificial skin; skin stimulation; spinal cord cells; Electric variables measurement; Electrophysiology; Fabrication; In vitro; Microchannel; Microstructure; Nervous system; Neurons; Skin; Spinal cord; Biomedical Transducers; Microsystem; Nervous System;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on

Print_ISBN

0-7803-8711-2

Type

conf

DOI

10.1109/MMB.2005.1548421

Filename

1548421