Tripodal thiol-derivatives as a functional interface monolayer for immobilization of biomolecules

We designed interface molecules for immobilization of biomolecules on solid substrate and applied it to genetic field effect transistors. We have been investigating a new approach to realize a potentiometric detection for DNA chips. The concept of a genetic field effect transistor has been proposed for improving precision, standardization and miniaturization of a DNA chip system. We have designed and synthesized tripodal thiol-derivatives for stable immobilization of oligonucleotide probes on a gold surface. The genetic FET platform combined with the new interface molecule is suitable for a simple, accurate and inexpensive system for SNP typing in clinical diagnostics.