Intrinsic optical signal imaging of a ratiometric fluorescence oxygen nanosensor

Author

Zhang, G. ; Shitole, P.S. ; Pujari, R.A. ; Charnani, V.S. ; McShane, M.J. ; Robinson, C.J.

Author_Institution

Biomed. Eng. Program, Louisiana Tech. Univ., Ruston, LA, USA

fYear

2005

fDate

12-15 May 2005

Firstpage

339

Lastpage

342

Abstract

Several imaging techniques and methods have been developed to map the metabolic or electrical changes during brain functions in high spatial and temporal resolutions. Wide-field intrinsic optical signal (IOS) imaging is an excellent method for its spatial resolution of 50-100 μm and a wide imaging field of several mm² that cannot be achieved by any microscope. A nano-encapsulated fluorescence dye sensor that is specifically sensitive to oxygen level was fabricated. The fluorescence images of the nanosensor were collected by a wide-field IOS imaging system under two different oxygen concentrations and analyzed by MATLAB software for the nanosensor´s oxygen quenching property.

Keywords

bioelectric potentials; biomedical optical imaging; brain; chemical sensors; chemical variables measurement; fluorescence; mathematics computing; medical computing; neurophysiology; spatiotemporal phenomena; MATLAB software; brain function; fluorescence image; nanoencapsulated fluorescence dye sensor; nanosensor oxygen quenching property; optical signal imaging; oxygen concentration; ratiometric fluorescence oxygen nanosensor; spatial resolution; temporal resolution; wide imaging field; Fluorescence; High-resolution imaging; Image analysis; Image resolution; MATLAB; Optical imaging; Optical microscopy; Optical sensors; Signal resolution; Spatial resolution; Electrostatic layer-by-layer (LbL) self-assembly; intrinsic optical signal (IOS) imaging; nanosensor;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on

Print_ISBN

0-7803-8711-2

Type

conf

DOI

10.1109/MMB.2005.1548467

Filename

1548467