Title :
Intrinsic optical signal imaging of a ratiometric fluorescence oxygen nanosensor
Author :
Zhang, G. ; Shitole, P.S. ; Pujari, R.A. ; Charnani, V.S. ; McShane, M.J. ; Robinson, C.J.
Author_Institution :
Biomed. Eng. Program, Louisiana Tech. Univ., Ruston, LA, USA
Abstract :
Several imaging techniques and methods have been developed to map the metabolic or electrical changes during brain functions in high spatial and temporal resolutions. Wide-field intrinsic optical signal (IOS) imaging is an excellent method for its spatial resolution of 50-100 μm and a wide imaging field of several mm2 that cannot be achieved by any microscope. A nano-encapsulated fluorescence dye sensor that is specifically sensitive to oxygen level was fabricated. The fluorescence images of the nanosensor were collected by a wide-field IOS imaging system under two different oxygen concentrations and analyzed by MATLAB software for the nanosensor´s oxygen quenching property.
Keywords :
bioelectric potentials; biomedical optical imaging; brain; chemical sensors; chemical variables measurement; fluorescence; mathematics computing; medical computing; neurophysiology; spatiotemporal phenomena; MATLAB software; brain function; fluorescence image; nanoencapsulated fluorescence dye sensor; nanosensor oxygen quenching property; optical signal imaging; oxygen concentration; ratiometric fluorescence oxygen nanosensor; spatial resolution; temporal resolution; wide imaging field; Fluorescence; High-resolution imaging; Image analysis; Image resolution; MATLAB; Optical imaging; Optical microscopy; Optical sensors; Signal resolution; Spatial resolution; Electrostatic layer-by-layer (LbL) self-assembly; intrinsic optical signal (IOS) imaging; nanosensor;
Conference_Titel :
Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on
Print_ISBN :
0-7803-8711-2
DOI :
10.1109/MMB.2005.1548467
