Development of a platform for microscale monitoring of cell function and differentiation

Author

Guenat, O.T. ; Generelli, S. ; Clerc, P.-A. ; de Rooij, N.F. ; Koudelka-Hep, M. ; Berthiaume, Francois ; Yarmush, M.L.

Author_Institution

Center for Eng. in Medicine, Harvard Med. Sch., Boston, MA, USA

fYear

2005

fDate

12-15 May 2005

Firstpage

343

Lastpage

344

Abstract

We present the technological development of a platform aimed for the monitoring of cell function and differentiation. The platform is equipped with an array of 16 silicon nitride micropipettes (diameter=3 μm), located at the bottom of a 350 μm deep microchannel, and with two microfluidic drug delivery systems placed on both sides of the microchannel. The micropipettes house ion selective microelectrodes and the microfluidics serve to deliver growth and differentiation factors to cells to be cultured at the bottom of the channels. We are currently developing such a system using NH₄⁺-selective microelectrodes to monitor the differentiation of stem cells into hepatocytes.

Keywords

biomedical electrodes; cellular biophysics; drug delivery systems; microchannel flow; microelectrodes; 3 micron; 350 micron; NH₄⁺; SiN; cell differentiation; cell function; hepatocytes; ion selective microelectrodes; microchannel; microfluidic drug delivery systems; microfluidics; microscale monitoring; silicon nitride micropipettes; Biochemistry; Biomedical monitoring; Drug delivery; Electrodes; Microchannel; Microelectrodes; Microfluidics; Pharmaceutical technology; Silicon; Stem cells; Ion-selective electrode; cell differentiation; local drug delivery; micropipette;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on

Print_ISBN

0-7803-8711-2

Type

conf

DOI

10.1109/MMB.2005.1548468

Filename

1548468