DocumentCode :
2650704
Title :
Inactivation of microorganisms by pulsed electric field treatment: the influence of pulse parameters on the inactivation rate
Author :
Frey, W. ; Gusbeth, C. ; Schwartz, T. ; Volkmann, H. ; Kirchen, S. ; Wuestner, R. ; Straessner, R. ; Bluhm, H.
Author_Institution :
Inst. fur Hochleistungsimpuls und Mikrowellentechnik, Forschungszentrum Karlsruhe, Eggenstein-Leopoldshafen
fYear :
2006
fDate :
4-8 June 2006
Firstpage :
364
Lastpage :
364
Abstract :
Summary form only given. For a bacterial decontamination of critical wastewater outlets like those from hospitals, conventional methods fail. In the presence of organic substances, ozonization or chlorination can produce toxic reagents. Also the penetration depth of UV radiation in thick water is insufficient. With regard to an industrial implementation of the pulsed electric field treatment technique the influence of the pulse parameters on the inactivation of Pm. putida and E. faecium was studied. The bacteria were suspended in diluted PBS-buffer at a conductivity of 0.2 Sm-1 and treated with rectangular pulses at a field strength of up to 125 kV cm-1 . The duration of the pulses was varied between 100 ns and 10 mus. For an energy of 120 kJ/l delivered to the bacterial suspension a maximum inactivation rate of 3.6 log rates was observed when 1 mus, 100 kV cm-1 pulses were applied. The inactivation rate decreases for shorter pulse widths. Compared to unipolar pulses, the application of fast reversing bipolar pulses did not improve the killing rate. A high sampling size study involving exponential pulses of different decay time and rectangular pulses results in similar killing rates for both pulse shape types
Keywords :
bioelectric phenomena; biological effects of fields; biological effects of ionising radiation; microorganisms; 100 to 10000 ns; E. faecium; Pm. putida; UV radiation; bacterial decontamination; bipolar pulses; chlorination; critical wastewater; exponential pulses; microorganism inactivation; organic substances; ozonization; penetration depth; pulsed electric field treatment; rectangular pulses; unipolar pulses; Conductivity; Decontamination; Hospitals; Microorganisms; Pulse shaping methods; Sampling methods; Shape; Space vector pulse width modulation; Wastewater treatment;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Plasma Science, 2006. ICOPS 2006. IEEE Conference Record - Abstracts. The 33rd IEEE International Conference on
Conference_Location :
Traverse City, MI
Print_ISBN :
1-4244-0125-9
Type :
conf
DOI :
10.1109/PLASMA.2006.1707237
Filename :
1707237
Link To Document :
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