DocumentCode :
2652614
Title :
Time-resolved fluorescence microscopy of stressed membranes of living endothelial cells
Author :
Butler, Peter J. ; Patterson, Brian ; Ferko, Michael ; Bowen, Benjamin P.
Author_Institution :
Dept. of Bioeng., Pennsylvania State Univ., University Park, PA, USA
Volume :
2
fYear :
2004
fDate :
7-10 Nov. 2004
Firstpage :
1826
Abstract :
Advances in the understanding of mechanotransduction in endothelial cells require reliable predictive models of stress and readouts of mechano-activation in the same cell. In order to determine correlations between stress and cellular activation, we have coupled rapid 3-D rendering of optical images of stained endothelial cells and computational fluid and mechanics models with photonics-based detection of molecular perturbation. Central to our approach is a multimodal, confocal molecular dynamics microscope with time-correlated, single-photon counting electronics to measure excited-state lifetime dynamics of fluorescent molecules in cell membranes. Preliminary results suggest a correlation of positive shear stress gradients and membrane tension with shear-induced membrane perturbations at sub-micron scales. Such position-dependence of membrane stress concentrations may be used as cues for mechano-chemical signal transduction.
Keywords :
biological techniques; cellular biophysics; fluorescence; optical images; optical microscopy; partial differential equations; photon counting; shear strength; cell mechanoactivation; cell membranes; confocal molecular dynamics microscope; endothelial cells; living endothelial cells; mechanochemical signal transduction; mechanotransduction; molecular perturbation; optical images; photonics-based detection; stressed membranes; time-resolved fluorescence microscopy; Biomembranes; Cells (biology); Electron microscopy; Fluid dynamics; Fluorescence; Optical computing; Optical coupling; Predictive models; Rendering (computer graphics); Stress;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Signals, Systems and Computers, 2004. Conference Record of the Thirty-Eighth Asilomar Conference on
Print_ISBN :
0-7803-8622-1
Type :
conf
DOI :
10.1109/ACSSC.2004.1399479
Filename :
1399479
Link To Document :
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