Title :
Photoporation and cell transfection using a violet diode laser
Author :
Paterson, L. ; Agate, B. ; Comrie, M. ; Ferguson, R. ; Lake, T.K. ; Morris, J.E. ; Carruthers, A.E. ; Brown, C.T.A. ; Sibbett, W. ; Bryant, P.E. ; Gunn-Moore, F. ; Riches, A.C. ; Dholakia, K.
Author_Institution :
Sch. of Phys. & Astron., Saint Andrews Univ.
Abstract :
Stable transfection of Chinese hamster ovary (CHO-K1) cells is achieved by photoporation with a violet diode laser. This represents the simplest method of laser-assisted cell poration reported to date
Keywords :
biological effects of laser radiation; biomembranes; cellular effects of radiation; genetics; semiconductor lasers; Chinese hamster ovary cell transfection; laser-assisted cell poration; photoporation; stable cell transfection; violet diode laser; Biomedical optical imaging; Biomembranes; Cells (biology); Chemical lasers; Diode lasers; Laser beams; Optical filters; Optical microscopy; Photonics; Ultrafast optics;
Conference_Titel :
Quantum Electronics and Laser Science Conference, 2005. QELS '05
Conference_Location :
Baltimore, MD
Print_ISBN :
1-55752-796-2
DOI :
10.1109/QELS.2005.1549238
