Title :
Quantitative Two-photon Flow Cytometry
Author :
Zhong, Cheng Frank ; Ye, Jing Yong ; Norris, Theodore B. ; Thomas, Thommey ; Myc, Andrzje ; Bielinska, Anna U. ; Baker, James R., Jr.
Author_Institution :
Center for Ultrafact Opt. Sci., Michigan Univ., Ann Arbor, MI
Abstract :
Two-photon excitation in conjunction with a suitable multi-dye labeling strategy enables quantitative flow cytometry under conditions of nonuniform flow, such as may be encountered in simple capillary flow or in vivo
Keywords :
biological fluid dynamics; biomedical measurement; cellular biophysics; channel flow; dyes; flow measurement; infrared spectroscopy; laser applications in medicine; measurement by laser beam; two-photon spectroscopy; capillary flow; femtosecond near-infrared laser; in vivo measurement; multidye labeling strategy; nonuniform flow; two-photon excitation; two-photon flow cytometry; Biomedical optical imaging; Fluorescence; Image motion analysis; Labeling; Laser excitation; Optical filters; Optical scattering; Size measurement; Ultrafast optics; Wavelength measurement;
Conference_Titel :
Quantum Electronics and Laser Science Conference, 2005. QELS '05
Conference_Location :
Baltimore, MD
Print_ISBN :
1-55752-796-2
DOI :
10.1109/QELS.2005.1549241
