Rapid detection of fibrinogen and fibrin degradation products by latex piezoelectric immunoassay

Author

Aizawa, Hidenobu ; Tozuka, Mitsuhiro ; Park, Jong-Won ; Noda, Kazutoshi ; Kobayashi, Koichi ; Reddy, Subrayal Medapati ; Kurosawa, Shigeru

Author_Institution

Nat. Inst. of Adv. Ind. Sci. & Technol., Japan

fYear

2003

fDate

4-8 May 2003

Firstpage

961

Lastpage

963

Abstract

It was developed a conventional immunosensor for fibrinogen and fibrin degradation products (FDP) to combine a quartz crystal microbalance (QCM) with the agglutination reaction of immunized latex beads. We successfully measured FDP concentration of in human serum within 10 min by QCM method. The detection range of QCM immunosensor is covered with screening concentration of FDP in serum (less than 10 μg/ml of FDP). The time course of latex agglutination obtained from QCM immunosensor is synchronized to that of latex photometric immunoassay. Frequency shift on immunoreaction explains the increased adsorption amount of agglutinated latex on QCM.

Keywords

adsorption; biomedical materials; biosensors; microbalances; piezoelectric devices; piezoelectric materials; piezoelectric oscillations; proteins; quartz; adsorption; agglutination reaction; fibrin degradation; fibrinogen; frequency shift; human serum; immunosensor; latex bead; latex photometric immunoassay; latex piezoelectric immunoassay; quartz crystal microbalance; screening concentration; Clinical diagnosis; Coagulation; Crystalline materials; Degradation; Electrodes; Frequency synchronization; Humans; Immune system; Medical diagnosis; Photometry;

fLanguage

English

Publisher

ieee

Conference_Titel

Frequency Control Symposium and PDA Exhibition Jointly with the 17th European Frequency and Time Forum, 2003. Proceedings of the 2003 IEEE International

ISSN

1075-6787

Print_ISBN

0-7803-7688-9

Type

conf

DOI

10.1109/FREQ.2003.1275220

Filename

1275220