DocumentCode
2660109
Title
Single live-bacterial cell assay of promoter activity and regulation: Escherichia coli gcl promoter
Author
Teramoto, Jun ; Yamanishi, Yoko ; Magdy, El-Shimy H. ; Hasegawa, Akiko ; Nakajima, Masahiro ; Shimada, Tomohiro ; Arai, Fumihito ; Fukuda, Toshio ; Ishihama, Akira
Author_Institution
Dept. of Frontier Biosci., Hosei Univ., Tokyo, Japan
fYear
2009
fDate
9-11 Nov. 2009
Firstpage
255
Lastpage
258
Abstract
For measurement of the promoter activity and regulation within single bacterial cells, we constructed various types of the cell chip. After gelation of portions of E. coli culture within the cell chip, we have succeeded, for the first time, the real-time single-bacterial cell assay of promoter activity. Here we applied this newly developed system for measurement of the activity and regulation of E. coli gcl promoter.
Keywords
biological techniques; cellular biophysics; microorganisms; Escherichia coli gcl promoter; cell chip; gelation; promoter activity; single live-bacterial cell assay; Bioinformatics; Genomics; Glass; Indium tin oxide; Lithography; Microelectrodes; Microorganisms; Polymers; RNA; Semiconductor device measurement;
fLanguage
English
Publisher
ieee
Conference_Titel
Micro-NanoMechatronics and Human Science, 2009. MHS 2009. International Symposium on
Conference_Location
Nagoya
Print_ISBN
978-1-4244-5094-7
Electronic_ISBN
978-1-4244-5095-4
Type
conf
DOI
10.1109/MHS.2009.5351949
Filename
5351949
Link To Document