Micro bubble manipulation towards single cell handling tool

We report a series of micro air bubble manipulations (transporting, merging and eliminating of air bubbles) in two-dimensional microchannels filled with a water solution. Air bubbles (~500 nano liters in volume) are driven by electrowetting-on-dielectric (EWOD) principle. By sequentially energizing an array of electrodes covered with dielectric layers, air bubbles can be transported along a programmed path, merged into a single larger one, and eliminated out of a bulk water solution. This bubble manipulation technique can be applied to efficiently handle micro objects. As a proof of concept, we successfully demonstrate in a millimeter scale that a fish egg and a sesame husk are attached to an air bubble and then driven by the bubble in a programmed way. The moving air bubble can push and pull the attached objects of which surface is hydrophilic or hydrophobic. This handling technique will bring a novel, versatile, cost-effective, high throughput tool, enabling us to efficiently transport, trap, and isolate individual micro bio-entities such as cells, functional particles and possibly molecules