Micro raleigh-benard convection polymerase chain reaction chip

Polymerase chain reaction (PCR) is a molecular biological method for in vitro amplification of nucleic acid molecule. In this paper, a micro Rayleigh-Benard convection PCR chip, consist of two constant temperature micro heaters, micro temperature sensors, and one PDMS reaction chamber, is developed. The chip is easy to be fabricated by using MEMS technology. Often seen micro reaction chamber PCR chip need excellent dynamic temperature control to increase the heating and cooling rate, and designed chamber to increase reaction efficiency, but Rayleigh-Benard convection PCR chip can solve these problem. With proper size of chamber design, the sample reagent in it can transit its reaction temperature immediately. Also, the faster the speed of flow is, the higher the heating and cooling rate is. At current stage, real DNA has been used to examine the amplification performance of developed biochip