Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.