A Novel HPLC Method for Determining Three Effective Components in Chinese Medicine ´Xiao´ Erjiebiao Granules

A simple, rapid and reproducible HPLC method was used for the simultaneous determination of chlorogenic acid, baicalin and forsythin in Xiao´erjiebiao Granules, at the same time, response surface methodology (RSM) was devoted to forecast the best extraction technology. The HPLC System included an Agilent ZORBAX SB-C18 column ( 250 mm × 4.6 mm, 5μm ), acetonitrile (A) - 0.4% ethylic acid (B) as mobile phase with gradient elution at a flow rate of 1.0 mL.min-1 and detection wavelength of 327 nm for chlorogenic acid, 280 nm for baicalin and forsythin, respectively. The good linearity was achieved successfully with R² <; 0.9995 in its standard curve aspects. Recoveries measured at the same concentrations (n = 6) were in the range of 96.4-97.6%. The established assay is accurate and practical, which could be utilized as a quality evaluation and control for Xiao´erjie biao Granules.