Parallelization offissionand fusion+ operations for high throughput generation of combinatorial droplets

We present a highly parallelized, programmable droplet-based device for high throughput generation of combinatorial droplets. The device features a multichannel, parallel architecture. Throughput is increased by two orders of magnitude compared to conventional devices [1]. With 32 Hz droplet generation, the projected throughput of this parallelized design is nearly 3 million sample-probe droplets per day on a single device (with 4 replicates of 750 thousand different mixtures). This translates to 240 unique sample-probe mixtures with 4 replicates per minute. This device has the potential to meet the need for low-cost, high throughput screening applications.