Drastic Enhancement of Maysin and Maysin Derivatives Contents in the Centipedegrass Extracts by Different Stresses

Maysin, a flavone C-glucoside, is only found the silks and leaves of maize, teosinte and centipede grass, which are resistant to the major insect pests such as the corn earworm and the fall armyworm in the Southeastern United States. It was recently reported various biological activities such as anti cancer and antioxidant. In spite of this importance, the investigation on may sin derivatives was carried out very limitedly because may sin and may sin derivatives are not able to synthesize chemically and the route for obtaining these compounds is very limited. The contents of luteolin were increased from 0.64 to 0.86, 1.00, and 1.29 μg/ml by gamma irradiation, UV-B irradiation, and wounding, respectively. The contents of isoorientin were increased from 41.72 to 91.05 and 119.50 μg/ml by UV-B irradiation and wounding. The contents of rhamnosylisoorientin and orient in were increased from 15.43 to 25.20 and 119.50 μg/ml by UV-B irradiation and wounding, respectively. By gamma irradiation, the contents of isoorientin and rhamnoslysioorientin were similar with red leaf. The contents of may sin were increased from 18.87 to 29.28, 22.45, and 32.57 μg/ml by gamma irradiation, UV-B irradiation, and wounding, respectively. The contents of derhamnosylmaysin were increased from 18.85 to 48.67, 58.54, and 93.65 μg/ml by gamma irradiation, UV-B irradiation, and wounding, respectively. The last, the contents of luteoin-6-Cboivinopyranose were increased from 1.00 to 2.07, 3.45, and 5.57 μg/ml by gamma irradiation, UV-B irradiation, and wounding, respectively. According to the results, the contents of may sin and may sin derivatives among various stresses, wounding treatment is the most promising method for increasing the contents of may sin and may sin derivatives and gamma irradiation and UV-B irradiation is effective method for increasing the contents of may sin and may sin derivatives.