Title :
The Effects of Curcumin on the Proteome of L02 Cells
Author :
Wang, Ping-Er ; Lu, De-Zhao ; Lin, Tao-Qi ; Wang, Ya-Li ; Wo, Xing-De
Author_Institution :
Coll. of Life Sci., Zhejiang Chinese Med. Univ., Hangzhou, China
Abstract :
Objective: To study the effects of curcumin on the proteome of L02 cells with the techniques of two-dimensional gel electrophoresis and mass-spectrometric. Methods: The total proteins of the untreated L02 cells and the L02 cells treated with 25 μmol/L curcumin for 24 hours were separated and the 2-DE was used to establish the proteome maps. The differential expression proteins between the two groups were identified with the MALDI-TOF-MS. Results: 8 differential expression proteins were found, in which heat shock protein 70, glucose-regulated protein 75, proliferating cell nuclear antigen, eukaryotic translation initiation factor-3, β-tubulin were up-regulated, and nuclear phosphoprotein-1, laminin receptor were down-regulated. Conclusion: These identified differential proteins might be related to the mechanism of the curcumin anti-atherosclerotic and anti-tumor.
Keywords :
cellular biophysics; desorption; electrophoresis; mass spectroscopy; molecular biophysics; proteins; proteomics; time of flight mass spectroscopy; tumours; β-tubulin; L02 cell proteoms; MALDI-TOF-MS analysis; antitumor mechanism; curcumin; curcumin antiatherosclerotic mechanism; differential expression proteins; eukaryotic translation initiation factor-3; glucose-regulated protein; heat shock protein; laminin receptor; mass-spectrometry; nuclear phosphoprotein-1; proliferating cell nuclear antigen; time 24 h; two-dimensional gel electrophoresis; Cancer; Cells (biology); Electric shock; Heating; Proteins; Tumors; 2-DE; Curcumin; L-02 cells; Mass spectrometry; Proteome;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.483
