Title :
The Study of Drug Resistance of C.Albicans Biofilm to Chinese Medicinal Herb Sophora Flavescens and Liquorice
Author :
Li, Jian ; Wang, Ailin ; Wang, Jihan
Author_Institution :
Sch. of Med. Lab. Sci., Beihua Univ. Jilin, Jilin, China
Abstract :
Objective: To study the drug resistance of C. albicans biofilm at different stage of C. albicans biofilm formation to Chinese medicinal herb Sophora flavescens and liquorice. Methods: The morphology of the biofilm at different development stages(2, 4, 8, 24, 48h)in glass slide was observed under the inverted microscope. The drug resistance of dissociative C. albicans was detect with NCCLs microdilution and the changes of C. albicans drug resistance in biofilm were tested with XTT reduction method. Results: Drug resistance of C.albicans increased with the biofilm maturation significantly ( P<;0.05). Compared with planktonic C. albicans strains, The drug resistance of biofilm is 100 times. Conclusion:There is positive correlation between drug resistance and biofilm maturation of C. albicans.
Keywords :
biochemistry; biomedical optical imaging; cellular biophysics; drugs; microorganisms; molecular biophysics; C. albicans biofilm; Chinese medicinal herb; NCCL microdilution; Sophora flavescens; XTT reduction method; biofilm maturation; biofilm morphology; drug resistance; inverted microscope; liquorice; Drugs; Immune system; Microorganisms; Microscopy; Morphology; Strain; Suspensions; C. albicans; XTT reduction method; biofilm; drug resistance;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.444
