UV Absorption Spectroscopic Study on the Interaction between Copper(II) Complex [Cu2Cl4(C17H22N2)2] and Bovine Serum Albumin

Author

Liu, Yu-Fen ; Xia, Hai-Tao ; Rong, De-Fu

Author_Institution

Sch. of Chem. Eng., Huaihai Inst. of Technol., Lianyungang, China

fYear

2012

fDate

28-30 May 2012

Firstpage

651

Lastpage

653

Abstract

The binding reaction of the Copper(II) Complex [Cu₂C₁₄(C₁₇H₂₂N₂)₂] with bovine serum albumin (BSA) was studied by UV absorption spectroscopy under the simulative physiological conditions. The binding constants were 6.67×10⁴, 5.61×10⁴ and 3.79×10⁴ L·mol^-1 at 291K, 298 K and 305K respectively. The thermodynamic parameters ΔH, ΔS and ΔG were calculated at different temperatures, and the result showed that hydrogen bonds and Van der Waals are two main reactions between Copper(II) Complex and BSA.

Keywords

bio-optics; biochemistry; copper compounds; hydrogen bonds; molecular biophysics; physiological models; proteins; reaction rate constants; thermodynamics; ultraviolet spectra; van der Waals forces; UV absorption spectroscopy; binding constant; binding reaction; bovine serum albumin; copper complex; hydrogen bonds; simulative physiological condition; thermodynamic parameter; van der Waals forces; Absorption; Bovine; Chemistry; Copper; Hydrogen; Spectroscopy; Thermodynamics; Bovine serum albumin; Cu(II) complex; UV absorption spectrum;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on

Conference_Location

Macau, Macao

Print_ISBN

978-1-4577-1987-5

Type

conf

DOI

10.1109/iCBEB.2012.463

Filename

6245203