A Hybrid Electrokinetic-Microfluidic Chip for Isolating CTCs in Physiological Sample

Author

Liu, Yu ; Song, Ningning ; Dang, Juan ; Chen, Hairong ; Yang, Dong ; Gao, Jian ; Song, Dajun ; Yang, Tianzhu ; Tian, Hongxiao

Author_Institution

Dept. of Chem., Shandong Polytech. Univ., Jinan, China

fYear

2012

fDate

28-30 May 2012

Firstpage

962

Lastpage

964

Abstract

This paper reports a hybrid electrokinetic-microfluidic chip, which combines electrophoresis (EP), dielectrophoresis (DEP) or AC electrothermal flow (ACEF), for isolating circulating tumor cells (CTCs) in physiological sample. While most electrokinetic techniques only function in low-conductivity buffers, hybrid electrokinetics enables effective operation in high-conductivity samples (1 S/m), which are compatible to physiological fluids such as blood, buffy coat and urine. Using a 3-electrode configuration and optimizing AC voltage, frequency and DC offset, we demonstrate that breast cancer cell can be localized within 10 sec in buffy coat without any pretreatment.

Keywords

bioMEMS; biomedical measurement; cancer; cellular biophysics; electrophoresis; lab-on-a-chip; microfluidics; tumours; AC electrothermal flow; AC voltage optimisation; ACEF; CTC isolation; DC offset optimisation; blood; breast cancer cell; buffy coat; circulating tumor cells; dielectrophoresis; frequency optimisation; high conductivity samples; hybrid electrokinetic-microfluidic chip; physiological sample; three electrode configuration; urine; Blood; Cancer; Electric fields; Electrodes; Electrokinetics; Microfluidics; Physiology; CTCs; bioprocessor; electrokinetic; microfluidic chip;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on

Conference_Location

Macau, Macao

Print_ISBN

978-1-4577-1987-5

Type

conf

DOI

10.1109/iCBEB.2012.27

Filename

6245282