Analysis of Sonoporation on Cell Membrane by Scanning Electron Microscopy

Sonoporation is an important drug/gene delivery technology in drug/gene targeting therapy. In this article, the fibroblast cell and red blood cell suspensions were added contrast agent micro bubbles and sonicated by continues low-intensity ultrasound, and then the pores on cell membrane were detected by scanning electron microscopy. It was found that the sonoporation on cell membrane was positive correlation with the ultrasound exposure power and ultrasound exposure time. And it was found that the sonoporation was related with cell membrane elastic characteristics. It provided experiment basement for the qualitative and quantitative research on sonoporation.