Title :
Photobleaching of dendrimer under multi-photon excitation
Author :
Hong, Xin ; Chang, Chifang ; Jiang, Chip ; Kao, Fu-Jen
Author_Institution :
Coll. of Precision Instrum. & Photo-electronics Eng., Tianjin Univ., China
Abstract :
Photobleaching as a result of intense laser irradiation has been an important issue in the applications of laser scanning microscopy. In this study we are employing time-lapsed techniques to study the effects of photobleaching of dendrimer under multi-photon excitation. We have found that multi-photon excitation have impacts on the samples in ways very different from cw illumination in the linear domain.
Keywords :
high-speed optical techniques; laser beam effects; multiphoton processes; optical materials; optical microscopy; optical saturable absorption; photoexcitation; cw illumination; dendrimer; intense laser irradiation; multiphoton excitation; multiphoton fluorescence laser scanning microscopy; photobleaching; time-lapsed techniques; Educational institutions; Fluorescence; Focusing; Laser excitation; Laser theory; Lighting; Microscopy; Optical pulse generation; Photobleaching; Power generation;
Conference_Titel :
Lasers and Electro-Optics, 2003. CLEO/Pacific Rim 2003. The 5th Pacific Rim Conference on
Print_ISBN :
0-7803-7766-4
DOI :
10.1109/CLEOPR.2003.1277135