Title :
The Effect of VHS Specific siRNA on Herpes Simplex Virus-1 Replication
Author :
Sun, Liyuan ; Liu, Limei ; Zhao, Yundong ; Yang, Ming
Author_Institution :
Sch. of Med. Lab., Beihua Univ. Jilin, Jilin, China
Abstract :
Objective: To explore the effects of siRNA targeting to Virion host shutoff protein (VHS) on HSV-1 replication in vero cell. Methods: Three kinds of siRNAs were transcripted in vitro using T7RNA polymerase according to the principle of siRNA design. The Vero cells were transfected with siRNA using lipofectamine 2000 followed by HSV-1 infection. The activity of vero cells were detected by MTT method and virus titer were monitored from 12h to 72h after infection with single step growth curve assay. Results: VHS-specific siRNAs were apparently able to decrease the dead numbers of vero cells infected by HSV-1, and the virus titer showed the apparent difference between two groups (P<;0.05), compared with the control group. Conclusions: siRNA synthesized in the study could inhibit viral replication and protect host cells through interfering VHS protein synthesis. The result provided a preliminary theory for treating HSV-1 by siRNA in the further.
Keywords :
RNA; biomedical measurement; cellular biophysics; enzymes; gene therapy; microorganisms; HSV-1 infection; HSV-1 replication; MTT method; T7RNA polymerase; VHS specific siRNA effects; herpes simplex virus-1 replication; in vitro transcription; lipofectamine 2000 followed; siRNA design; siRNA targeting; single step growth curve assay; vero cell; viral replication inhibition; virion host shutoff protein; virus titer; Educational institutions; Immune system; Interference; Polymers; Proteins; RNA; Viruses (medical); HSV-1; VHS; siRNA; viral replication;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.417
