• DocumentCode
    2685179
  • Title

    Induction and Selection of Stenotrophomonas maltophilia DHHJ for Feather Degradation

  • Author

    Wu, Xiaoqian ; Wang, Jing ; Zhuang, Yuan ; Cao, Zhangjun ; Zhou, Meihua

  • Author_Institution
    Dept. of Environ. Sci. & Eng., Donghua Univ., Shanghai, China
  • fYear
    2012
  • fDate
    28-30 May 2012
  • Firstpage
    1521
  • Lastpage
    1524
  • Abstract
    Stenotrophomonas maltophilia DHHJ, a new feather-degrading bacterium, was subjected to mutation by ultraviolet light for 90 s. The mutant L2 was selected due to its greater activity in comparison with the wild strain. After incubation at 40°C for 6 days, the feathers degraded by mutant L2 were 1.3-fold more than S. maltophilia DHHJ, and the keratinase was secreted. After cultivation for 6 days in 5 L fermenter with 50% air saturation, the rate of feather degradation was 92.3% and the activity of keratinase produced was 8.6 U/mL by mutant L2. The results proved that mutant L2 had high genetic stability and could be possibly used in the industrial processes.
  • Keywords
    cellular biophysics; enzymes; genetics; microorganisms; molecular biophysics; Stenotrophomonas maltophilia DHHJ; air saturation; feather-degrading bacterium; genetic stability; incubation; industrial processes; keratinase activity; mutant L2; temperature 40 degC; time 6 d; time 90 s; ultraviolet light mutation; Degradation; Feathers; Microorganisms; Proteins; Radiation effects; Strain; Ultraviolet light; degradation; feather; mutation;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
  • Conference_Location
    Macao
  • Print_ISBN
    978-1-4577-1987-5
  • Type

    conf

  • DOI
    10.1109/iCBEB.2012.248
  • Filename
    6245424