DocumentCode
2685179
Title
Induction and Selection of Stenotrophomonas maltophilia DHHJ for Feather Degradation
Author
Wu, Xiaoqian ; Wang, Jing ; Zhuang, Yuan ; Cao, Zhangjun ; Zhou, Meihua
Author_Institution
Dept. of Environ. Sci. & Eng., Donghua Univ., Shanghai, China
fYear
2012
fDate
28-30 May 2012
Firstpage
1521
Lastpage
1524
Abstract
Stenotrophomonas maltophilia DHHJ, a new feather-degrading bacterium, was subjected to mutation by ultraviolet light for 90 s. The mutant L2 was selected due to its greater activity in comparison with the wild strain. After incubation at 40°C for 6 days, the feathers degraded by mutant L2 were 1.3-fold more than S. maltophilia DHHJ, and the keratinase was secreted. After cultivation for 6 days in 5 L fermenter with 50% air saturation, the rate of feather degradation was 92.3% and the activity of keratinase produced was 8.6 U/mL by mutant L2. The results proved that mutant L2 had high genetic stability and could be possibly used in the industrial processes.
Keywords
cellular biophysics; enzymes; genetics; microorganisms; molecular biophysics; Stenotrophomonas maltophilia DHHJ; air saturation; feather-degrading bacterium; genetic stability; incubation; industrial processes; keratinase activity; mutant L2; temperature 40 degC; time 6 d; time 90 s; ultraviolet light mutation; Degradation; Feathers; Microorganisms; Proteins; Radiation effects; Strain; Ultraviolet light; degradation; feather; mutation;
fLanguage
English
Publisher
ieee
Conference_Titel
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location
Macao
Print_ISBN
978-1-4577-1987-5
Type
conf
DOI
10.1109/iCBEB.2012.248
Filename
6245424
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