Isolation, Culture and Cryopreservation of Sika Deer Antler Dermis Layer Cells

To study the biological characteristics of sika deer antler dermis layer cells, the dermis layer of growing tip in sika deer antler was collected and a culture and cryopreservation system in vitro for the dermis layer cells was established. The results showed that the antler dermis layer cells could be cultured well within a short term in DMEM medium with 10% FBS in vitro. The cultured dermis layer cells were spindle or rhombic and the cultured cells grew to confluence after 5 days. The cells before the fourth generation appeared uniform. The edge of the cells was smooth and the cytoplasm was uniform and transparent. The cultured cells were closely arranged. The cultured cells changed into flat after the fifth generation and extended more apophysises. The edge of the cultured cells was not smooth and the cells were loosely arranged after merged. The suitable cryopreserved condition for the dermis layer cells was DMEM medium containing 5% DMSO and 10% FBS with stepwise freezing.