Purification of Antitumor Polysaccharides from Mushroom Fermentation Broth by Macroporous Resins

Medicinal mushroom polysaccharides from submerged culture have lots of important biological activities, such as antitumor, immunomodulating, antioxidation and so on. In order to improve the biological activities of the polysaccharides, pigments impurities produced in the fermentation process have to be eliminated by a suitable technology before polysaccharides purification and application. 28 kinds of macro porous resins were investigated in this study and the results showed that macro porous resin OU-1 had satisfactory pigment elimination ability. After optimization by uniform design and verification experiments, the optimal condition for pigments adsorption by macro porous resin OU-1 was found. The optimized conditions were that the fermentation supernatant with pH ranging from 5.2 to 5.8 could be directly applied to pigments adsorption for 3.9 h at 40°C. At the optimized condition, about eighty percent of the pigment in fermentation supernatant could be eliminated, ninety-five percent of the polysaccharides in the fermentation supernatant could be recovered, and only about 41mg/ml of the resin (wet weight/v) was needed.