Title :
Cell lysisa using surface acoustic wave devices for sensor applications
Author :
Lyford, T.J. ; Millard, P.J. ; da Cunha, M. Pereira
Author_Institution :
Chem. & Biol. Eng. Dept., Univ. of Maine, Orono, ME, USA
Abstract :
This paper reports on the use of surface acoustic wave (SAW) devices for lysis of the Gram-positive bacterium Geobacillus thermoglucosidasius. Vegetative cells were exposed to surface acoustic wave energy for up to 8 min at 5° to 55°C. DNA concentration was quantified by fluorescence, and quantitative PCR was performed to confirm molecular recognition of specific DNA sequences. The successful use of SAW devices for lysis of bacterial cells is reported for the first time in this work, opening the possibility of using SAW as a lysing mechanism in portable DNA detection devices.
Keywords :
DNA; biosensors; cellular biophysics; enzymes; fluorescence; microorganisms; molecular biophysics; molecular configurations; portable instruments; surface acoustic wave sensors; surface acoustic waves; DNA concentration; SAW devices; cell lysis; fluorescence; gram-positive bacterium Geobacillus thermoglucosidasius; molecular recognition; portable DNA detection devices; quantitative polymerase chain reaction; sensor applications; specific DNA sequences; surface acoustic wave devices; surface acoustic wave energy; temperature 5 degC to 55 degC; time 8 min; vegetative cells; DNA; Microorganisms; Probes; Surface acoustic wave devices; Surface acoustic waves; DNA detection; acoustic wave lysis; acoustic wave microfluidics;
Conference_Titel :
Ultrasonics Symposium (IUS), 2012 IEEE International
Conference_Location :
Dresden
Print_ISBN :
978-1-4673-4561-3
DOI :
10.1109/ULTSYM.2012.0303
