DocumentCode :
2690993
Title :
Perfusion estimation using 3D subharmonic imaging: An in vivo study
Author :
Sridharan, Arun ; Eisenbrey, J.R. ; Machado, P. ; Ji-Bin Liu ; Suhyun Park ; Dianis, Scott ; Wallace, K. ; Thomenius, K.E. ; Forsberg, F.
Author_Institution :
Dept. of Radiol., Thomas Jefferson Univ., Philadelphia, PA, USA
fYear :
2012
fDate :
7-10 Oct. 2012
Firstpage :
1102
Lastpage :
1105
Abstract :
Under sufficient acoustic excitation microbubble-based ultrasound contrast agents (UCA) produce a marked subharmonic (f0/2) frequency component. By selectively receiving at the subharmonic, it is possible to perform UCA specific subharmonic imaging (SHI). We investigated the ability to quantify tissue perfusion (in ml/min/g) in 5 canines using 3D SHI. A Logiq 9 scanner (GE Healthcare, Milwaukee, WI) equipped with a 4D10L probe was modified to perform 3D harmonic imaging (HI; ftransmit=5 MHz and freceive=10 MHz) and 3D SHI (ftransmit=5.8 MHz and freceive=2.9 MHz). Imaging was performed in the lower renal pole of open-abdomen canines after injection of Definity (Lantheus Medical Imaging, N Billerica, MA). The canines received a 5 μl/kg bolus injection of Definity for HI and a 20 μl/kg for SHI in triplicate for each kidney. A microvascular staining technique based on isotope-labeled microspheres was used to quantify the degree of perfusion in each kidney. Ligation of a surgically exposed branch of the renal arteries induced low perfusion. Time-intensity curves were generated from regions-of-interest (ROIs) corresponding to the tissue samples used for microvascular staining. Perfusion was estimated from the initial slope of the fractional blood volume uptake and compared to the reference standard using linear regression analysis. Microsphere perfusion data showed an average perfusion of 9.30±6.60 and 5.15±3.42 ml/min/g pre and post ligation, respectively. The reference standard showed significant correlation with 3D HI perfusion estimates (r=0.38; p=0.007), but correlated better with 3D SHI (r=0.62; p<;0.001). Additionally, these results showed an improvement 2D SHI perfusion estimates previously reported by our group (r=0.57; Forsberg et al JUM 2006). In conclusion, 3D SHI perfusion estimates were in better overall agreement with the reference standard than 3D HI and were superi- r to previously reported 2D SHI results.
Keywords :
biomedical ultrasonics; blood vessels; haemodynamics; haemorheology; kidney; ultrasonic imaging; 3D subharmonic imaging; Definity; Logiq 9 scanner; acoustic excitation microbubble; fractional blood volume uptake; frequency 10 MHz; frequency 2.9 MHz; frequency 5 MHz; frequency 5.8 MHz; isotope labeled microspheres; kidney; lower renal pole; microvascular staining technique; open abdomen canine; perfusion estimation; subharmonic frequency component; time-intensity curves; tissue perfusion; ultrasound contrast agents; Acoustics; Blood; Correlation; Harmonic analysis; Imaging; Kidney; Ultrasonic imaging; Ultrasound contrast agents; perfusion estimation; subharmonic imaging;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Ultrasonics Symposium (IUS), 2012 IEEE International
Conference_Location :
Dresden
ISSN :
1948-5719
Print_ISBN :
978-1-4673-4561-3
Type :
conf
DOI :
10.1109/ULTSYM.2012.0274
Filename :
6562199
Link To Document :
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