X-ray diffraction, transmission electron microscopy and Rutherford backscattering/channeling are used to characterize the structure of an InGaN/GaN multiple quantum well (MQW) grown on an Al
2O
3(0001) substrate. Using the combination of these measurements, not only the layered structure of the MQW is revealed clearly but also the In content x (0.16) of the In
xGa
1-N component can be determined reliably, which is a prerequisite for further determination of the elastic strain of the MQW. X-ray θ-2θ scans of the (0002) and (101~4) diffractions are used to determine the average lattice parameters
epi> and epi> of the MQW, consequently the average parallel elastic strain 11> = -0.25% and the average perpendicular elastic strain ⊥> = +0.13% of the MQW are deduced. The experimental result of ⊥>/11> = -0.52 is in good agreement with the value deduced from the elastic constants.