Fluorescence lifetime imaging with measurements of photon migration: theory, simulation, and experiment

Author

Sevick-Muraca, E.M. ; Reynolds, J.S. ; Troy, T.L. ; Lopez, G. ; Paithankar, D.Y. ; Lee, J.

Author_Institution

Sch. of Chem. Eng., Purdue Univ., West Lafayette, IN, USA

Volume

6

fYear

1997

fDate

30 Oct-2 Nov 1997

Firstpage

2729

Abstract

Frequency-domain measurements of photon migration are coupled with a model of fluorescence generation and propagation in order to develop a method for reconstructing maps of fluorescent properties within interior tissue volumes from exterior measurements at the air-tissue interface. Simulated results confirm the feasibility of optical imaging through the use of exogenously administered contrast agents on the basis of fluorophore decay kinetics and yield. Experimental measurements using single-pixel and multi-pixel devices illustrate that the contrast from exogenous fluorescence exceeds that owing to absorption or scattering caused by endogenous chromophores or tissue structure and owing to absorption caused by exogenous contrast agents. The prognosis for fluorescence lifetime imaging as a prognostic imaging modality is discussed

Keywords

biomedical imaging; fluorescence; frequency-domain analysis; image reconstruction; inverse problems; medical image processing; optical tomography; photon transport theory; radiative lifetimes; tumours; turbidity; air-tissue interface; amplitude modulation; exogenously administered contrast agents; exterior measurements; fluorescence generation model; fluorescence lifetime imaging; fluorophore decay kinetics; frequency-domain measurements; image reconstruction; interior tissue volumes; inverse problem; multi-pixel devices; multiply scattered light; optical imaging; phase shift difference; photon migration; prognostic imaging modality; quantum yield; reconstructing maps; simulation; single-pixel devices; tumours; turbid media; Absorption; Fluorescence; Frequency measurement; Image reconstruction; Kinetic theory; Optical imaging; Optical propagation; Optical scattering; Particle scattering; Volume measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1997. Proceedings of the 19th Annual International Conference of the IEEE

Conference_Location

Chicago, IL

ISSN

1094-687X

Print_ISBN

0-7803-4262-3

Type

conf

DOI

10.1109/IEMBS.1997.756896

Filename

756896