Title :
Cell membrane electroporation-Part 3: the equipment
Author :
RebersÌŒek, M. ; Miklavcic, Damijan ; Bertacchini, C. ; Sack, M.
Author_Institution :
Lab. of Biocybernetics, Univ. of Ljubljana, Ljubljana, Slovenia
Abstract :
As described in Part 1, a cell membrane can be made permeable to various molecules by carrying out a procedure called electroporation [1]. This procedure is being successfully used in biology, biotechnology, and medicine [2], [3]. It requires electroporators and electrodes. An electroporator generates short HV pulses of specific shape, amplitude, duration, number, and repetition frequency [4], and the pulses are applied to the target cells or load through the electrodes [5]. The energy delivered to the load is governed by the number of pulses and the pulse voltage, current, and duration. In biomedical applications that energy can be several joules; in biotechnology, where electroporation is used for treatment of agricultural products and water, it can be several kilojoules.
Keywords :
biomedical electrodes; biomembranes; cellular biophysics; high-voltage techniques; molecular biophysics; pulse generators; agricultural products treatment; biology; biomedical application; biotechnology; cell membrane electroporation; electrodes; electroporator; medicine; pulse current; pulse duration; pulse voltage; short HV pulse generation; water treatment; Cell membranes; Electrodes; Generators; Impedance; Optical switches; Power transmission lines; Pulse generation; Safety; electrode; electroporator; pulse generator; safety standard;
Journal_Title :
Electrical Insulation Magazine, IEEE
DOI :
10.1109/MEI.2014.6804737