Title :
Investigation methods of cell attachment on porous silicon matrix
Author :
Miu, Mihaela ; Angelescu, Anca ; Kleps, Irina ; Petrescu, Stefana ; Zdrentu, Livia ; Craciunoiu, Florea ; Simion, Monica ; Ignat, Teodora ; Bragaru, Adina
Author_Institution :
Nat. Inst. for R&D in Microtechnol., Bucharest, Romania
Abstract :
Different porous silicon layers, with a large pore size distribution were realised on p+Si (100), followed by thermal treatments for structure surface modification/stabilisation in order to ensure the PS biocompatibility. On these structures, murine melanomas (B16 - F1), (B16-F10) and hamster ovarian cells (CHO) were grown, and studied by different investigation methods: scanning electron microscopy (SEM), and optical microscopy for the pore morphologies investigation, fluorescence microscopy for the cell/surface occupancy investigation and fluorescence spectroscopy to study the adhesion process and the interaction of cells with the substrate.
Keywords :
adhesion; biomolecular electronics; fluorescence spectroscopy; heat treatment; molecular biophysics; optical microscopy; porous semiconductors; scanning electron microscopy; surface phenomena; surface treatment; PS biocompatibility; adhesion process; cell attachment; cell interaction; cell occupancy; fluorescence microscopy; fluorescence spectroscopy; hamster ovarian cells; murine melanomas; optical microscopy; p+Si (100); pore morphologies; pore size distribution; porous silicon layers; porous silicon matrix; scanning electron microscopy; structure surface modification; structure surface stabilisation; surface occupancy; thermal treatments; Biomedical optical imaging; Electron optics; Fluorescence; Malignant tumors; Optical microscopy; Scanning electron microscopy; Silicon; Spectroscopy; Surface morphology; Surface treatment;
Conference_Titel :
Semiconductor Conference, 2004. CAS 2004 Proceedings. 2004 International
Print_ISBN :
0-7803-8499-7
DOI :
10.1109/SMICND.2004.1402803
