DocumentCode :
2713789
Title :
Optical detection of photo-activated protein adsorption using Brewster angle microscopy
Author :
Li, Y. ; Song, H.Y. ; Lear, M.J. ; Gorelik, S. ; Tomczak, N. ; Hobley, J.
Author_Institution :
Dept. of Chem., Nat. Univ. of Singapore, Singapore
fYear :
2008
fDate :
8-11 Dec. 2008
Firstpage :
1
Lastpage :
4
Abstract :
Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266 nm light yields the pi-pi* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, in-situ using a Brewster angle microscope (BAM).
Keywords :
adsorption; optical microscopy; photochemistry; proteins; Brewster angle microscope; Brewster angle microscopy; benzophenone derivatives; bovine serum albumin; buffer solution; deposition; immobilization; ketyl radical; optical detection; photo-reactions; photoactivated protein adsorption; pi-pi* triplet state; pulsed laser irradiation; quartz substrate; silane coupling; Bovine; Laser excitation; Monitoring; Optical buffering; Optical coupling; Optical detectors; Optical microscopy; Optical pulses; Proteins; Protons;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
PhotonicsGlobal@Singapore, 2008. IPGC 2008. IEEE
Conference_Location :
Singapore
Print_ISBN :
978-1-4244-3901-0
Electronic_ISBN :
978-1-4244-2906-6
Type :
conf
DOI :
10.1109/IPGC.2008.4781381
Filename :
4781381
Link To Document :
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