A dual-excitation difference technique for fluorescence rejection in Raman spectroscopy

Fluorescence disturbance is the primary limiting factor of biomedical Raman spectroscopy. This paper proposed a dual-excitation difference technique for fluorescence rejection. In this research, a low-cost dual-excitation system was built for Raman detection, and a robust deconvolution algorithm was developed for spectrum recovery. Simulation and experimental results demonstrated that this method could effectively eliminate the fluorescence background in Raman spectroscopy.