Title :
Generation of Oxidative Stress in Cells by Localized Laser Radiation
Author :
He, Hao ; Kong, Siu-Kai ; Lee, Rebecca Kit-Ying ; Chan, Kam Tai
Author_Institution :
Dept. of Electron. Eng., Chinese Univ. of Hong Kong, Hong Kong
Abstract :
Localized laser beams had been observed to generate reactive oxygen species (ROS) in human hepatocellular carcinoma cells (HepG2). The oxidative stress and thermal effect generated depended on the nature of the laser beam used. It was found that the ROS-induced fluorescence signal caused by femtosecond (fs) laser pulses were much smaller than that due to continuous-wave laser radiation around 1550nm. This implies fs laser pulses are relatively harmless to cells.
Keywords :
biological effects of laser radiation; cellular biophysics; fluorescence; high-speed optical techniques; laser applications in medicine; ROS-induced fluorescence signal; continuous-wave laser radiation; femtosecond laser pulses; human hepatocellular carcinoma cells; localized laser radiation; oxidative stress; reactive oxygen species; thermal effect; Cells (biology); Fiber lasers; Fluorescence; Humans; Laser beams; Microscopy; Optical pulses; Power lasers; Stress; Ultrafast optics;
Conference_Titel :
PhotonicsGlobal@Singapore, 2008. IPGC 2008. IEEE
Conference_Location :
Singapore
Print_ISBN :
978-1-4244-3901-0
Electronic_ISBN :
978-1-4244-2906-6
DOI :
10.1109/IPGC.2008.4781389
