Title :
Quantification of collagen alteration in mid-dermis of skin lesion with mucinosis using second harmonic generation imaging
Author :
Lin, Lihang ; Zhu, Xiaoqin ; Yu, Haibo ; Chen, Jianxin
Author_Institution :
Key Lab. of Optoelectron. Sci. & Technol. for Med. of Minist. of Educ., Fujian Normal Univ., Fuzhou, China
Abstract :
Cutaneous mucinoses are a heterogeneous group of diseases characterized by abnormal deposition of dermal mucin. Considering the collagen disorder due to the mucin deposition, collagen microstructure was visualized by second harmonic generation (SHG) microscopy to gain structural and diagnostic information. High resolution images of collagen in the dermis of skin lesion with mucinosis and healthy skin showed differences in their microstructures. SHG density as well as depth dependant decay coefficient was analyzed to quantitatively characterize the microstructure. Our results demonstrated the possibility of SHG imaging to in vivo tracking the pathological process of mucinoses and evaluate the therapeutic efficacy.
Keywords :
biomedical optical imaging; diseases; optical harmonic generation; optical microscopy; proteins; skin; SHG microscopy; abnormal dermal mucin deposition; collagen alteration quantification; collagen disorder; collagen microstructure; cutaneous mucinoses; depth dependent decay coefficient; diagnostic information; high resolution collagen images; mucinosis; second harmonic generation imaging; skin lesion middermis; structural information; therapeutic efficacy; Aging; Laboratories; Lesions; Microscopy; Skin; Three dimensional displays;
Conference_Titel :
Bioelectronics and Bioinformatics (ISBB), 2011 International Symposium on
Conference_Location :
Suzhou
Print_ISBN :
978-1-4577-0076-7
DOI :
10.1109/ISBB.2011.6107670
